why Repeat send the email when I restart My Web Server ?

hi,
I have a web server (IIS 5.0,VS.NET 1.0,WIN 2000 SERVER ) and I also installed the SMTP
service.now I have an asp.net application,and in my asp.net page I can Send email successful(I checked: c:\Inetpub\mailroot\queue ,there have lots of Internet Email message)
but there has a problem:

when I restarted my web server,the server will automicaly send the email where stored in
c:\inetpub\mailroot\queue.

I tried to stop smtp service and remove the email from c:\inetpub\mailroot\queue,But the server will show:
Access Deny,the File is in Used.

I know my smtp server configruation has problem,but I don't know where it is .

Anybody can give me a solution ?

thanks in advanced!You can try to restart your server IIS and the machine itself, else try to uninstall SMTP and then install it again.

regards
yes,I have been done it before,but still have the same problem.